Evaluation of the genotoxic activity of paclitaxel by the in vitro micronucleus test in combination with fluorescent in situ hybridization of a DNA centromeric probe and the alkaline single cell gel electrophoresis technique (comet assay) in human T-lymphocytes

1999 ◽  
Vol 34 (4) ◽  
pp. 269-278 ◽  
Author(s):  
L. Digue ◽  
T. Orsi�re ◽  
M. De M�o ◽  
M. G. Matt�i ◽  
D. Depetris ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
T Lymphocytes ◽  
Comet Assay ◽  
Gel Electrophoresis ◽  
Fluorescent In Situ Hybridization ◽  
Micronucleus Test ◽  
Single Cell Gel Electrophoresis ◽  
Electrophoresis Technique

scholarly journals Combination of Direct Viable Count and Fluorescent In Situ Hybridization (DVC-FISH) as a Potential Method for Identifying Viable Vibrio parahaemolyticus in Oysters and Mussels

Foods ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1502
Author(s):  
Jorge García-Hernández ◽  
Manuel Hernández ◽  
Yolanda Moreno
Keyword(s):  
In Situ Hybridization ◽  
Vibrio Parahaemolyticus ◽  
Fluorescent In Situ Hybridization ◽  
Potential Method ◽  
Viable Count ◽  
Hybridization Technique ◽  
Fish Technique ◽  
Viable Cells

Vibrio parahaemolyticus is a human food-borne pathogen with the ability to enter the food chain. It is able to acquire a viable, non-cultivable state (VBNC), which is not detected by traditional methods. The combination of the direct viable count method and a fluorescent in situ hybridization technique (DVC-FISH) makes it possible to detect microorganisms that can present VBNC forms in complex samples The optimization of the in vitro DVC-FISH technique for V. parahaemolyticus was carried out. The selected antibiotic was ciprofloxacin at a concentration of 0.75 μg/mL with an incubation time in DVC broth of 5 h. The DVC-FISH technique and the traditional plate culture were applied to detect and quantify the viable cells of the affected pathogen in artificially contaminated food matrices at different temperatures. The results obtained showed that low temperatures produced an important logarithmic decrease of V. parahaemolyticus, while at 22 °C, it proliferated rapidly. The DVC-FISH technique proved to be a useful tool for the detection and quantification of V. parahaemolyticus in the two seafood matrices of oysters and mussels. This is the first study in which this technique has been developed to detect viable cells for this microorganism.

Comparison of in vitro and in situ genotoxicity in the Danube River by means of the comet assay and the micronucleus test

2010 ◽  
Vol 700 (1-2) ◽  
pp. 11-17 ◽  
Author(s):  
Melanie Boettcher ◽  
Stefanie Grund ◽  
Steffen Keiter ◽  
Thomas Kosmehl ◽  
Georg Reifferscheid ◽  
...  
Keyword(s):  
Comet Assay ◽  
Micronucleus Test ◽  
Danube River ◽  
The Danube River
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